Pairwise detection of site-specific receptor phosphorylations using single-molecule blotting
نویسندگان
چکیده
منابع مشابه
Pairwise detection of site-specific receptor phosphorylations using single-molecule blotting
Post-translational modifications (PTMs) of receptor tyrosine kinases (RTKs) at the plasma membrane (PM) determine the signal transduction efficacy alone and in combination. However, current approaches to identify PTMs provide ensemble results, inherently overlooking combinatorial PTMs in a single polypeptide molecule. Here, we describe a single-molecule blotting (SiMBlot) assay that combines bi...
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ژورنال
عنوان ژورنال: Nature Communications
سال: 2016
ISSN: 2041-1723
DOI: 10.1038/ncomms11107